Caterina Romei was born in Florence in 1960 and she has lived there her entire life. In 1986 she completed her Laurea in Art History at the University of Florence with a thesis on the history of the church of the Santissima Annunziata in Florence.

In addition to the courses she teaches for Santa Reparata International School of Art, she has taught art history for several foreign university programs and schools for foreigners in Florence, including Washington University, Goshen College, the Koinè School, the Emily School of Art and Design, and others. In 2004 she completed a specialized thesis in Museum Studies at the University of Florence, writing a thesis about visitors and copyists in the Uffizi during the late eighteenth century.